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Showing posts with label development series. Show all posts
Showing posts with label development series. Show all posts

Monday, May 9, 2011

Matsuyama x Oishi (GoldfishGarage) top view Ranchu

Matsuyama Female (Hater 08) x Oishi Male (Oishi 06) 53 days post spawn.
Same spawning even and female as the:
Matsuyama x Itoh spawn used in the Ranchu Development Series.  Squares are 2 cm each side or 1.1 inches diagonal.


 

Thursday, May 5, 2011

Transparent Bristol Shubunkin Goldfish 60 days post spawn

See them from the beginning.
These are the homozygous culls out of a Bristol Shubunkin spawn.  They are half the size of the calico heterozygotes and a third the size of the homozygous wild type siblings.  This is my second species to develop/isolate a transparent fish in, a world's first?  Here is my first transparent fish in a zebrafish I call absolute.  I've sent it to reachers in 18 countries.  I came to the concept of a metallic eyed transparent zebrafish while thinking about these fish and how weak they are with their all black eyes.  Hopefully the absolute goldfish will be useful to science also.  

Matsuyama x Itoh 3/11 Ranchu fry 48 dpf

48 days post spawn.
See them from minutes after the spawn to current here:

Thursday, April 28, 2011

Thursday, March 17, 2011

Matsuyama x Itoh (Lains) 3/11 Development Series

23C, 8.0 pH and 3 ppt Salt.

16 Cells
16 Cells


Natural spawn on moss


In vitro (squeeze) on plate


Already developing

 

Sunday, March 6, 2011

Bristol Shubunkin (Goldfish Garage) 3/6/11: Development Series

Bristol Shubunkin Squeeze:





5 dpf swim bladder inflated 

Floating Fry Tub.

4.5 days old
4.5 days post fertilization (dpf) heart and gill arches.


84 hpf whole fry view.

84 hpf fry plate.

50 hours post fertilization group shot.

50 hours post fertilization side view.


50 hours post fertilization Dorsal view.


Ventral view of 36 hour old fry which is part of the Bristol Shubunkin 

Dorsal view of 36 hour old fry which is part of the Bristol Shubunkin 


24 hpf. The man in the moon.

24 hpf

20 hpf



A back view.

10 hpf

10 hpf


Bad egg.

5:30 pm Sunday 6 March 2011
5 hpf


5 hours post fertilization.
Midgefly larva on 5 hpf Bristol Shubunkin embryos.
You can tell it is an insect by it's eyes, antennae and legs.

Digiscoped through nikon e200 with an iPhone 4.



12:30 Sunday 6 March 2011
Very small but well documentable Bristol Shubunkin squeeze/spawn. They were spawning and eating their eggs till noon when I went to feed the fish. Hand spawned a small/tiny wildtype female to two males: an orange and black nacreous and a fairly good calico.

A new photo to add to the favorite picture file.
Will have to retake with a plate full of eggs.

Black and orange male below the petri dish.

Adults floating in a 20 inch pan floating in a plywood tub with the 6 inch petri floating in the pan.



Here is how I hand spawn Bristol Shubunkins.  Sorry the camera mount kept falling but you get the idea.  This was a small spawn of approximately 100 embryos.  When the plate hatched there were 3 bad eggs.


Sunday, May 9, 2010

Mutant Black Ranchu Fry Videos



Mutant Black Ranchu Fry

These are 85 hour old black ranchu lethal recessive mutants.

A group of mutants.

Large Yolk.

Ventral Curve.

Dorsal Curve.

Sea Squirt.

Ball of Cells.

Normal Fry 85 hpf


83 hpf Hatched

83 hpf hatching and hatched


Infertile egg with Saprolegnia hyphae

This is a black Ranchu egg that wa not fertilized. The saprolignia
hyphae can be seen growing from the egg. http://www.wikipedia.org/wiki/Saprolegnia